Genotypic analysis of E. coli strains isolated from patients with cystitis and pyelonephritis

Urinary tract infection is the most common health problem affecting millions of people each year, mainly caused by a large genetically heterogeneous group of Escherichia coli called uropathogenic E. coli. This study investigates the genotypic analysis of E. coli strains isolated from patients with cystitis and pyelonephritis. During 2008-2009, 90 E. coli strains were analyzed, consisting of 48 isolates causing pyelonephritis in children and 42 isolates causing cystitis. Having identified the strains by standard methods, they were subtyped by pulsed field gel electrophoresis [PFGE] and their corresponding patterns were compared using dendrogram. Sixty five PFGE profiles were obtained from the genome of E. coli strains by this genotyping method. Thirty six and thirty three patterns were obtained for pyelonephritis and cystitis, respectively. Most strains exhibited twelve and thirteen bands and the patterns with eight or nineteen bands had the lowest rate. Genome sizes of the strains were between 1610-4170 kbp. With due attention to these results, genetic patterns showed that the strains had different clonalities and it could be suggested in some cases that the strains causing pyelonephritis or cystitis have common patterns and different diseases could be explained by different gene factors

[Comparison of genetic patterns of E. coli strains isolated from patients with cystitis and pyelonephritis, using Pulsed Field Gel Electrophoresis]

Urinary tract infection [UTI] is one of the most frequently acquired bacterial infections caused by a large genetically heterogeneous group of Escherichia coli which are called uropathogenic E. coli [UPEC]. Cystitis and pyelonephritis are two most common symptoms seen in patients with UTI. The genetic diversity of this organism has hampered the identification of UTI strains and it is unclear whether all UPEC isolates are capable of causing both cystitis and pyelonephritis. Therefore, Careful selection of appropriate genotyping methods is mandatory. The most popular method is Pulsed Field Gel Electrophoresis [PFGE] that is used in the present study to evaluate the genetic patterns of UPEC. In this cross-sectional study a total of 90 E. coli strains consisting of 48 isolates causing pyelonephritis and 42 isolates causing cystitis in children were analyzed by PFGE and their corresponding patterns were compared. Sixty six PFGE profiles were obtained from the genome of E. coli strains by this genotyping method. Most strains exhibited twelve and thirteen bands and the patterns with eight or nineteen bands had the lowest rate. Genome size of strains was between 1610-4170 kbp. According to these results, it can be suggested that in some cases the strains causing pyelonephritis or cystitis have common patterns and different clinical symptoms could be attributed to different gene factors

[Discrimination of dominant H. pylori strains isolated from patients with different gastroduodenal pathologies by protein profiling]

It is not clear what factors determine divergent outcomes of infections caused by H. pylori. The aim of this study was to differentiate H. pylori strains isolated from the patients with different gastroduodenal pathologies by protein profiling. The protein profiles of different strains of H. pylori isolated from 3 groups of patients with ulcerative disease, nonulcerative gastritis and cancer disease were analyzed using 1D-SDS-PAGE. Based on the highly divergent protein patterns, the similarity of the strains inside each group was 75%, 76.47% and 78.57% for cancerous, ulcerative and no ulcerative groups respectively, while about 30.76% of the protein bands were common in all strains isolated from three groups of the patients. Some of the observed bands were significantly specific for each group. We speculated that some H. pylori strains might be more associated with a specific disease than others, leading to the clustering of some, but not all, strains within each disease group. This study showed that protein profile can be a criterion used for discriminating of dominant states in different gastric clinical states. Specific and dominant proteins of different strains isolated from three groups of patients under the study could be welcome candidates for further exploration to be used both for laboratory tests, which analyze disease-specific H. pylori strains, and for diagnosis of different diseases and outcomes associated with this widespread bacterium